Time-Lapse Microscopy of Streptomyces coelicolor Growth and Sporulation
نویسندگان
چکیده
منابع مشابه
Time-lapse microscopy of Streptomyces coelicolor growth and sporulation.
Bacteria from the genus Streptomyces are among the most complex of all prokaryotes; not only do they grow as a complex mycelium, they also differentiate to form aerial hyphae before developing further to form spore chains. This developmental heterogeneity of streptomycete microcolonies makes studying the dynamic processes that contribute to growth and development a challenging procedure. As a r...
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Peptidoglycan is a major cell wall constituent of gram-positive bacteria. It is a dynamic macromolecule that is actively remodeled to enable cell growth and differentiation through a tightly choreographed interplay of hydrolytic and biosynthetic enzyme activities. The filamentous bacterium Streptomyces coelicolor has a complex life cycle that likely requires considerable cell wall remodeling to...
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INTRODUCTION By their very nature, biological systems are dynamic, and a proper understanding of the cellular and molecular processes underlying living organisms and how to manipulate them is a prerequisite to combat diseases and improve human health care. One of the major challenges of current biomedical research, therefore, is to unravel not just the spatial organization of these complex syst...
متن کاملWhiD and WhiB, homologous proteins required for different stages of sporulation in Streptomyces coelicolor A3(2).
The whiD locus, which is required for the differentiation of Streptomyces coelicolor aerial hyphae into mature spore chains, was localized by map-based cloning to the overlap between cosmids 6G4 and D63 of the minimal ordered library of Redenbach et al. (M. Redenbach et al., Mol. Microbiol. 21:77-96, 1996). Subcloning and sequencing showed that whiD encodes a homologue of WhiB, a protein requir...
متن کاملEffects of Metals on Streptomyces coelicolor Growth and Actinorhodin Production.
Actinorhodin production by Streptomyces coelicolor was used as a model system to study the effects of metals on growth and polyketide synthesis in a streptomycete. Numerous metals were tested in cultures grown in liquid media. Mercury and cadmium were highly toxic, and copper, nickel, and lead were less so, but all tended to inhibit both growth and antibiotic synthesis to a similar extent. Unex...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2008
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01233-08